产品货号：
GS4878
中文名称：
重组人G-CSF
英文名称：
Recombinant Human Granulocyte Colony Stimulating Factor
产品规格：
2μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 18.7kDa,a single non-glycosylated polypeptide chain containing 174 amino acids.
储存条件：冷冻（-20℃）
概述
Granulocyte colony stimulating factor(G-CSF)is a pleiotropic cytokine.It is mainly produced by monocytes and macrophages upon activation by endotoxin,TNF-α and IFN-γ.Besides,many other cell types can secreted this protein after LPS,IL-1 or TNF-α activation,which are fibroblasts,endothelial cells,astrocytes and bone marrow stromal cells.Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.G-CSF is cytokine that acts in hematopoiesis by controlling the production,differentiation,and function of 2 related white cell populations of the blood,the granulocytes and the monocytes-macrophages.In addition it may function in some adhesion or recognition events at the cell surface.In humans,two distinct cDNA clones for G-CSF,encoding 207 and 204 amino acid(a.a.)precursor proteins,have been isolated.Both proteins have a 30 a.a.signal peptide and have identical amino acid sequences except for a three a.a.insertion(deletion)at the 35th a.a.residue from the N-terminus of the mature protein.Human G-CSF is 73%identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人G-CSF，G-CSF, Human
储存条件：冷冻（-20℃）
概述
Granulocyte colony stimulating factor(G-CSF)is a pleiotropic cytokine.It is mainly produced by monocytes and macrophages upon activation by endotoxin,TNF-α and IFN-γ.Besides,many other cell types can secreted this protein after LPS,IL-1 or TNF-α activation,which are fibroblasts,endothelial cells,astrocytes and bone marrow stromal cells.Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.G-CSF is cytokine that acts in hematopoiesis by controlling the production,differentiation,and function of 2 related white cell populations of the blood,the granulocytes and the monocytes-macrophages.In addition it may function in some adhesion or recognition events at the cell surface.In humans,two distinct cDNA clones for G-CSF,encoding 207 and 204 amino acid(a.a.)precursor proteins,have been isolated.Both proteins have a 30 a.a.signal peptide and have identical amino acid sequences except for a three a.a.insertion(deletion)at the 35th a.a.residue from the N-terminus of the mature protein.Human G-CSF is 73%identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞98%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1ng/ml,corresponding to a specific activity of>1.0×107 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in 10mMsodium acetate buffer,containing 5%trehalose,pH 4.0. |
| 内毒素 | Less than 1 EU/μg of rHuG-CSF as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP |
相关搜索：重组人G-CSF，G-CSF, Human